http://hdl.handle.net/10027/8351 Thu, 23 May 2013 18:40:16 GMT 2013-05-23T18:40:16Z http://hdl.handle.net/10027/8596 Prenuptial Dental Extractions in Acadian Women: First Report of a Cultural Tradition Gordon, Sara C.; Kaste, Linda M.; Barasch, Andrei; Safford, Monika M.; Foong, Choong; ElGeneidy, Adry Background: Prenuptial tooth extractions, extractions of all teeth in at least one dental arch before marriage, are not identified in the dental literature. Driven by a professional encounter, the purpose of this study was to confirm the existence of this practice among Acadian women. Methods: An 8-item survey instrument with space for comments was mailed to 182 dentists from traditionally Acadian regions of Canada. The survey was provided in English and French. Results: Ninety dentists responded (50.3%); 8 of them (9%) had been asked to perform prenuptial extractions, and an additional 9 volunteered awareness of this practice. Awareness and requests were associated with dental practice in a county with a ‡ 20% French-speaking population. Conclusions: Prenuptial extractions in this population have been confirmed by the current cohort of dentists. The potential public health, clinical, and systemic health research implications for women who are edentulous for most of their adult life merit further study. Additionally, it is important to determine if interventions are needed to curtail cultural expectations of such practices. This is a copy of an article published in the Journal of Women's Health © 2011 copyright Mary Ann Liebert, Inc.; Journal of Women's Health is available online at: http://www.liebertonline.com. DOI:10.1089/jwh.2011.3074. Thu, 01 Dec 2011 06:00:00 GMT http://hdl.handle.net/10027/8596 2011-12-01T06:00:00Z http://hdl.handle.net/10027/8368 FoxM1 mediates the progenitor function of type II epithelial cells in repairing alveolar injury induced by Pseudomonas aeruginosa Liu, Yuru; Sadikot, Ruxana T.; Adami, Guy R.; Kalinichenko, Vladimir V.; Pendyala, Pendyala; Natarajan, Viswanathan; Zhao, You-yang; Malik, Asrar B. The alveolar epithelium is composed of the flat type I cells comprising 95% of the gas-exchange surface area and cuboidal type II cells comprising the rest. Type II cells are described as facultative progenitor cells based on their ability to proliferate and trans-differentiate into type I cells. In this study, we observed that pneumonia induced by intratracheal instillation of Pseudomonas aeruginosa (PA) in mice increased the expression of the forkhead transcription factor FoxM1 in type II cells coincidentally with the induction of alveolar epithelial barrier repair. FoxM1 was preferentially expressed in the Sca-1+ subpopulation of progenitor type II cells. In mice lacking FoxM1 specifically in type II cells, type II cells showed decreased proliferation and impaired trans-differentiation into type I cells. Lungs of these mice also displayed defective alveolar barrier repair after injury. Expression of FoxM1 in the knockout mouse lungs partially rescued the defective transdifferentiation phenotype. Thus, expression of FoxM1 in type II cells is essential for their proliferation and transition into type I cells and for restoring alveolar barrier homeostasis after PA-induced lung injury. © 2011 Liu et al. This article is distributed under a Creative Commons License(Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/ by-nc-sa/3.0/). DOI: 10.1084/jem.20102041 Fri, 01 Jul 2011 05:00:00 GMT http://hdl.handle.net/10027/8368 2011-07-01T05:00:00Z http://hdl.handle.net/10027/7842 Analysis of RNA from Brush Cytology Detects Changes in B2M, CYP1B1 and KRT17 Levels with OSCC in Tobacco Users Kolokythas, Antonia; Schwartz, Joel L.; Pytynia, Kristen B.; Panda, Suchismita; Yao, Mike; Homann, Brian; Sroussi, Herve Y.; Epstein, Joel B.; Gordon, Sara C.; Adami, Guy R. RNA expression analysis of oral keratinocytes can be used to detect early oral cancer but a limitation is the inability to obtain high quality RNA from oral tissue without using biopsies. While oral cytology cell samples can be obtained from patients in a minimally invasive manner they have not been validated for quantitative analysis of RNA expression. Earlier we showed RNA from brush cytology of hamster Oral Squamous Cell Carcinoma (OSCC) showed differential expression of B2M and CYP1B1 using real time RT-PCR in a Dibenz[a,I]pyrene, tobacco carcinogen, induced model of this disease. Here we show reproducibility of this approach to measuring gene expression in humans. Cytology brush samples from 12 tobacco and betel related OSCC and 17 nonmalignant oral lesions revealed B2M mRNA was enriched in tumor samples while CYP1B1 mRNA was reduced, similar to what was seen in the model system. Additionally, we showed that KRT17 mRNA, a gene linked to OSCC in another brush cytology study, was also enriched in OSCC versus nonmalignant lesions, again supporting the promise of using RNA from brush oral cytology to reproducibly monitor oral gene expression. NOTICE: this is the author’s version of a work that was accepted for publication in Oral Oncology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Oral Oncology, [Vol 47, Issue 6, (June 2011)] DOI: 10.1016/j.oraloncology.2011.03.029. The original publication is available at www.elsevier.com. Wed, 01 Jun 2011 05:00:00 GMT http://hdl.handle.net/10027/7842 2011-06-01T05:00:00Z