http://hdl.handle.net/10027/7825 Thu, 23 May 2013 09:25:00 GMT 2013-05-23T09:25:00Z http://hdl.handle.net/10027/8604 Identification of Microbial and Proteomic Biomarkers in Early Childhood Caries Hart, Thomas C.; Corby, Patricia M.; Hauskrech, Milos; Ryu, Ok Hee; Pelikan, Richard; Valko, Michal; Oliveira, Maria B.; Hoehn, Gerald T.; Bretz, Walter A. The purpose of this study was to provide a univariate and multivariate analysis of genomic microbial data and salivary massspectrometry proteomic profiles for dental caries outcomes. In order to determine potential useful biomarkers for dental caries, a multivariate classification analysis was employed to build predictive models capable of classifying microbial and salivary sample profiles with generalization performance. We used high-throughput methodologies including multiplexed microbial arrays and SELDI-TOF-MS profiling to characterize the oral flora and salivary proteome in 204 children aged 1–8 years (n = 118 cariesfree, n = 86 caries-active). The population received little dental care and was deemed at high risk for childhood caries. Findings of the study indicate that models incorporating both microbial and proteomic data are superior to models of only microbial or salivary data alone. Comparison of results for the combined and independent data suggests that the combination of proteomic and microbial sources is beneficial for the classification accuracy and that combined data lead to improved predictive models for caries-active and caries-free patients. The best predictive model had a 6% test error, >92% sensitivity, and >95% specificity. These findings suggest that further characterization of the oral microflora and the salivary proteome associated with health and caries may provide clinically useful biomarkers to better predict future caries experience. Copyright © 2011 Thomas C. Hart et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. DOI:10.1155/2011/196721 Sat, 01 Jan 2011 06:00:00 GMT http://hdl.handle.net/10027/8604 2011-01-01T06:00:00Z http://hdl.handle.net/10027/8160 The Effects of 17-beta Estradiol on Enhancing Proliferation of Human Bone Marrow Mesenchymal Stromal Cells In Vitro Hong, Liu; Zhang, Guoquan; Sultana, Habiba; Yu, Yang; Wei, Zhen Human bone marrow mesenchymal stromal cells (MSCs) with self-renewal and multiple differentiation potentials are considered a possible cell source for tissue engineering and regenerative medicine. However, the limited amount of MSCs in bone marrow and the loss of differentiation capacity following in vitro expansion restrict their practical application. Effective improvement of MSC proliferation is necessary for the clinical application of MSC-based tissue engineering. The effects of estrogen supplements on proliferation and characterizations of human MSCs were investigated at the present study. Supplements of 17-beta estradiol (E2) significantly increase the proliferation of human MSCs in vitro. The dose range of E2 to significantly increase MSC proliferation differs in the gender of MSC donor. E2 supplementation in cell proliferation maintains characterizations of MSCs, including cell surface markers, and osteogenic and adipogenic differentiation capacities. These data indicate that estrogen treatment can play an important role in improving human MSCs' expansion in vitro, which will effectively facilitate MSCs' function in the practical application of tissue engineering and regeneration. This is a copy of an article published in the Stem Cells and Development © 2011 Copyright Mary Ann Liebert, Inc.; Stem Cells and Development is available online at: http://www.liebertonline.com. DOI: 10.1089/scd.2010.0125 Sun, 01 May 2011 05:00:00 GMT http://hdl.handle.net/10027/8160 2011-05-01T05:00:00Z