http://hdl.handle.net/10027/7311 Sun, 19 May 2013 07:44:44 GMT 2013-05-19T07:44:44Z http://hdl.handle.net/10027/7842 Analysis of RNA from Brush Cytology Detects Changes in B2M, CYP1B1 and KRT17 Levels with OSCC in Tobacco Users Kolokythas, Antonia; Schwartz, Joel L.; Pytynia, Kristen B.; Panda, Suchismita; Yao, Mike; Homann, Brian; Sroussi, Herve Y.; Epstein, Joel B.; Gordon, Sara C.; Adami, Guy R. RNA expression analysis of oral keratinocytes can be used to detect early oral cancer but a limitation is the inability to obtain high quality RNA from oral tissue without using biopsies. While oral cytology cell samples can be obtained from patients in a minimally invasive manner they have not been validated for quantitative analysis of RNA expression. Earlier we showed RNA from brush cytology of hamster Oral Squamous Cell Carcinoma (OSCC) showed differential expression of B2M and CYP1B1 using real time RT-PCR in a Dibenz[a,I]pyrene, tobacco carcinogen, induced model of this disease. Here we show reproducibility of this approach to measuring gene expression in humans. Cytology brush samples from 12 tobacco and betel related OSCC and 17 nonmalignant oral lesions revealed B2M mRNA was enriched in tumor samples while CYP1B1 mRNA was reduced, similar to what was seen in the model system. Additionally, we showed that KRT17 mRNA, a gene linked to OSCC in another brush cytology study, was also enriched in OSCC versus nonmalignant lesions, again supporting the promise of using RNA from brush oral cytology to reproducibly monitor oral gene expression. NOTICE: this is the author’s version of a work that was accepted for publication in Oral Oncology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Oral Oncology, [Vol 47, Issue 6, (June 2011)] DOI: 10.1016/j.oraloncology.2011.03.029. The original publication is available at www.elsevier.com. Wed, 01 Jun 2011 05:00:00 GMT http://hdl.handle.net/10027/7842 2011-06-01T05:00:00Z http://hdl.handle.net/10027/7619 Deregulation of manganese superoxide dismutase (SOD2) expression and lymph node metastasis in tongue squamous cell carcinoma Liu, Xiqiang; Wang, Anxun; Muzio, Lorenzo L.; Kolokythas, Antonia; Sheng, Shihu; Rubini, Corrado; Ye, Hui; Shi, Fei; Yu, Tianwei; Crowe, David L.; Zhou, Xiofeng Background: Lymph node metastasis is a critical event in the progression of tongue squamous cell carcinoma (TSCC). The identification of biomarkers associated with the metastatic process would provide critical prognostic information to facilitate clinical decision making. Previous studies showed that deregulation of manganese superoxide dismutase (SOD2) expression is a frequent event in TSCC and may be associated with enhanced cell invasion. The purpose of this study is to further evaluate whether the expression level of SOD2 is correlated with the metastatic status in TSCC patients. Methods: We first examined the SOD2 expression at mRNA level on 53 TSCC and 22 normal control samples based on pooled-analysis of existing microarray datasets. To confirm our observations, we examined the expression of SOD2 at protein level on an additional TSCC patient cohort (n = 100), as well as 31 premalignant dysplasias, 15 normal tongue mucosa, and 32 lymph node metastatic diseases by immunohistochemistry (IHC). Results: The SOD2 mRNA level in primary TSCC tissue is reversely correlated with lymph node metastasis in the first TSCC patient cohort. The SOD2 protein level in primary TSCC tissue is also reversely correlated with lymph node metastasis in the second TSCC patient cohort. Deregulation of SOD2 expression is a common event in TSCC and appears to be associated with disease progression. Statistical analysis revealed that the reduced SOD2 expression in primary tumor tissue is associated with lymph node metastasis in both TSCC patient cohorts examined. Conclusions: Our study suggested that the deregulation of SOD2 in TSCC has potential predictive values for lymph node metastasis, and may serve as a therapeutic target for patients at risk of metastasis. The original version is available through BioMed Central at DOI: 10.1186/1471-2407-10-365. © 2010 Liu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fri, 09 Jul 2010 05:00:00 GMT http://hdl.handle.net/10027/7619 2010-07-09T05:00:00Z http://hdl.handle.net/10027/7353 Bone Healing in a Critical-Size Defect Using Platelet-Rich Plasma Miloro, Michael; Haralson, David J.; Desa, Valmont PURPOSE: To evaluate the effect of platelet-rich plasma (PRP) on bone healing in an osteotomized defect of the rabbit mandible. MATERIALS AND METHODS: Twelve adult female New Zealand White rabbits were randomized to 1 of 2 treatment groups: group A had an osteotomy with the addition of a bone graft, and group B had an osteotomy without a bone graft. Regardless of treatment group, 1 side in each rabbit was randomly selected to receive PRP as an internal control. Bilateral 1.0- x 0.5-cm mandibular inferior border osteotomies were performed in each animal, 0.5 cm anterior to the antegonial notch to create mandibular defects. The osteotomy sites were evaluated by histologic and radiographic analyses for bone healing at 1, 2, and 3 months after surgery. RESULTS: A 4-point ordinal scale was used to compare healing, based on radiographic density, radiographic height, and histologic height of new bone formation. Group A rabbits showed significantly shorter healing times compared with group B rabbits. A pairwise analysis indicated that the addition of PRP did not increase the overall score of any measured parameter, at any interval (P > .9). CONCLUSIONS: In the rabbit osteotomy model, bone grafting (group A) significantly improved healing in comparison with no bone grafting (group B). In defects of the mandible, an increased radiographic and histologic bone density and height were seen at 1-, 2-, and 3-month intervals in the bone graft group; however, the addition of PRP did not appear to provide any statistically significant benefit to healing in either group. Post print version of article may differ from published version. The definitive version is available through Elsevier at DOI: 10.1016/j.joms.2009.09.090 Tue, 01 Jun 2010 05:00:00 GMT http://hdl.handle.net/10027/7353 2010-06-01T05:00:00Z